Separation of standard proteins - stability after treatment with alkaline eluent
Summary
Type: HPLC Application #: 108220
Detection: UV, 280nm Conditions: eluent A: 0.1% TFA in H2O, eluent B: 0.08% TFA in CH3CN, gradient from 20 to 60% B in 10min flow rate 1.0ml/min Authors: LC department Source: Macherey-Nagel
Details
Legend: Separation of standard proteins
1. ribonuclease
2. cytochrome c
3. lysozyme
4. b-lactoglobulin
5. ovalbumin
Left: before treatment, right: after treatment with 300 column volumes of the alkali